Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays

A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat w...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 7; no. 12; pp. 896 - 901
Main Authors Wroczynski, Piotr, Wierzchowski, Jacek, Golab, Jakub, Blazejczyk, Magdalena, Borecka, Dorota
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.12.2002
MDPI
Subjects
Aldehyde dehydrogenase
Dehydrogenases
fluorimetric assays
Immunology
isozymes
Liver cancer
Oxidation
Proteins
Surgery
western blotting
Poland
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Summary:A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay.
ISSN:1420-3049
1420-3049
DOI:10.3390/71200896