Micropropagation of Oregano (Origanum vulgare x applii) from Meristem Tips

'Mendocino' oregano (Origanum vulgare x applii) cultivated in Argentina has been subject to progressive yield loss as a consequence of continuous vegetative propagation. A method of reducing damage to the shoot tip during sterilization procedures for micropropagation is proposed. Single sh...

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Bibliographic Details
Published inIn vitro cellular & developmental biology. Plant Vol. 39; no. 2; pp. 125 - 128
Main Authors Goleniowski, M. E., Flamarique, C., Bima, P.
Format Journal Article
LanguageEnglish
Published Wallingford CABI Publishing 01.03.2003
Cambridge University Press
Springer Nature B.V
Subjects
Agronomy. Soil science and plant productions
apical meristems
benzyladenine
Biological and medical sciences
Biotechnology
Culture media
Economic plant physiology
Fundamental and applied biological sciences. Psychology
In vitro culture
in vitro regeneration
Internodes
Meristems
Micropropagation
naphthaleneacetic acid
Oregano
Origanum applii
Origanum vulgare
Plant physiology and development
Plant propagation
Plant tissues
Plantlets
Plants
Protocols/Methods
Seeds
tissue culture
Tissue cultures, protoplasts
Argentina
Oregano; meristem tip culture; micropropagation
Labiatae
Dicotyledones
Angiospermae
Flavoring crop
Spermatophyta
Micropropagation
growth
Experimental protocol
Meristem
Origanum vulgare
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Summary:'Mendocino' oregano (Origanum vulgare x applii) cultivated in Argentina has been subject to progressive yield loss as a consequence of continuous vegetative propagation. A method of reducing damage to the shoot tip during sterilization procedures for micropropagation is proposed. Single shoot cuttings are less sensitive to disinfecting substances than green tips. Meristems taken from young plantlets that grew in vitro produced less oxidation during the culture than those dissected immediately after disinfecting from plants grown in the field. This work describes the effects of the growth regulators benzyladenine (BA) and naphthaleneacetic acid (NAA) at various concentrations and combinations on micropropagation of O. vulgare x applii. Treatment with 0.28 µM BA and 0.53 µM NAA gave greatest efficiency (mean: 22.2 nodes per plantlet). After 60 d 100% of rooted plantlets could be formed per explant under optimum conditions.
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ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2002361