Increased high-density lipoprotein-3 binding to leukocytes following weight loss and improved glycemic control in type 2 diabetic patients

• Published in: Metabolism, clinical and experimental Vol. 49; no. 6; pp. 692 - 697
• Main Authors: Paniagua, J.A., López-Miranda, J., Jansen, S., Zambrana, J.L., López Segura, F., Jiménez Perepérez, J.A., Pérez-Jiménez, F.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.06.2000; Elsevier
• Subjects: Adult; Biological and medical sciences; Blood Glucose - metabolism; Carrier Proteins; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Granulocytes - metabolism; Humans; Iodine Radioisotopes; Lipids - blood; Lipoproteins, HDL - blood; Lipoproteins, HDL3; Male; Medical sciences; Middle Aged; Protein Binding; Receptors, Lipoprotein - blood; RNA-Binding Proteins; Endocrinopathy; Human; Binding capacity; Pathophysiology; Cholesterol HDL; Treatment efficiency; Granulocyte; Apolipoprotein AI; Adult; Lipoprotein HDL; Non insulin dependent diabetes; Blood plasma
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1053/meta.2000.6248

This study evaluates the effect on high-density lipoprotein (HDL) binding activity in cultured granulocytes before and after metabolic control of non—insulin-dependent diabetes mellitus ([NIDDM] type 2 diabetes) patients. In 20 type 2 diabetic patients, diabetic control was accomplished by administration of oral antidiabetic agents and dietary restrictions. Adequate metabolic control was reflected by a decrease in the fasting glucose, glycosylated hemoglobin (HbA 1c), mean insulin, and body mass index (BMI). After control of the diabetes, the mean HDL 3 cholesterol was increased from 0.918 ± 0.05 to 1.008 ± 0.05 mmol/L ( P < .05) and apolipoprotein Al (apo Al) was increased from 103 ± 5.8 to 115 ± 5.1 mg/dL ( P < .01). The HDL 3 maximum specific binding was higher after versus before diabetic control, 77 ± 6 versus 122 ± 8 ng/mg cell protein ( P < .01). This increase was related to an increase in maximum binding ([B max ] from 4.97 × 10 −10 to 8.3 × 10 −10 mol/L, P < .001), and no significant changes were observed in the K d (from 1.47 × 10 −7 v 2.04 × 10 −7 mol/L). These results suggest that the metabolic control of type 2 diabetes increases HDL 3 binding activity.