Polymorphisms rs2167444 and rs508384 in the SCD1 Gene Are Linked with High ApoB-48 Levels and Adverse Profile of Cardiometabolic Risk Factors

• Published in: Folia biologica Vol. 65; no. 4; pp. 159 - 169
• Main Authors: Staňková, B, Macášek, J, Zeman, M, Vecka, M, Tvrzická, E, Jáchymová, M, Slabý, A, Žák, A
• Format: Journal Article
• Language: English
• Published: Czech Republic Charles University in Prague, First Faculty of Medicine 01.01.2019
• Subjects: Adult; Aged; Alleles; Apolipoprotein B; Apolipoprotein B-48 - blood; Apolipoprotein B-48 - metabolism; Apolipoproteins; Blood pressure; Body fat; Cardiovascular disease; Cardiovascular Diseases - blood; Cardiovascular Diseases - genetics; Cholesterol; Diabetes; Enzymes; Ethidium bromide; Fatty acids; Female; Gene expression; Genotype; Humans; Insulin resistance; Insulin Resistance - genetics; Intestine; Laboratories; Low density lipoprotein; Male; Metabolic syndrome; Middle Aged; Obesity; Oxidative stress; Oxidative Stress - genetics; Oxidative Stress - physiology; Polymorphism, Single Nucleotide - genetics; Risk Factors; Scd1 protein; Statistical analysis; Stearic acid; Stearoyl-CoA Desaturase - genetics; Studies; Triglycerides; Weight control
• ISSN: 0015-5500; 2533-7602
• DOI: 10.14712/fb2019065040159

Elevated plasma concentration of apolipoprotein B-48 (apoB-48) is an independent risk factor of cardiovascular disease. Stearoyl-CoA desaturase-1 (SCD1) is a rate-limiting lipogenic enzyme and a key regulator of fuel metabolism. The aim of this study was to analyse associations between clinical, biochemical, and genetic factors and different apoB-48 levels in subjects at increased cardiometabolic risk. We examined 220 subjects exhibiting at least one metabolic syndrome (MetS) component. In conjunction with basic clinical, anthropometric and laboratory measurements, we analysed various polymorphisms of stearoyl-CoA desaturase-1 (SCD1). Subjects were divided into two groups according to the median apoB-48 level: (1) high apoB-48 (≥ 7.9 mg/l, N = 112) and (2) low apoB-48 (< 7.9 mg/l, N = 108). Neither group differed significantly in anthropometric measures. High plasma apoB-48 levels were associated with increased systolic blood pressure (+3 %; P < 0.05), MetS prevalence (59.8 vs. 32.4 %; P < 0.001), small-dense LDL frequency (46.4 vs. 20.4 %; P < 0.001), triglycerides (+97 %; P < 0.001), non-HDLcholesterol (+27 %; P < 0.001), and lower concentrations of HDL-cholesterol (-11 %; P < 0.01). This group was further characterized by a higher HOMA-IR index (+54 %; P < 0.001) and increased concentrations of conjugated dienes (+11 %; P < 0.001) and oxidatively modified LDL (+ 38 %; P < 0.05). Lower frequencies of SCD1 minor genotypes (rs2167444, rs508384, P < 0.05) were observed in subjects with elevated plasma concentrations of apoB-48. Elevated plasma concentrations of apoB-48 are associated with an adverse lipid profile, higher systolic blood pressure, insulin resistance, and oxidative stress. Lower proportions of minor SCD1 genotypes (rs2167444, rs508384) implicate the role of genetic factors in the pathogenesis of elevated levels of apoB-48.