Multipotent Neurospheres Can Be Derived from Forebrain Subependymal Zone and Spinal Cord of Adult Mice after Protracted Postmortem Intervals

The adult mammalian CNS harbors a population of multipotent stem/progenitor cells that can be induced to grow as proliferative neurospheresin vitro.We demonstrate here that neurosphere-generating cells can be isolated from adult mouse spinal cord and forebrain subependymal zone after postmortem inte...

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Published inExperimental neurology Vol. 156; no. 2; pp. 430 - 433
Main Authors Laywell, Eric D., Kukekov, Valery G., Steindler, Dennis A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.1999
Subjects
Animals
Cell Survival
Cells, Cultured
DNA Replication
extended postmortem intervals
Mice
Mice, Inbred ICR
neural stem and progenitor cells
neural stem cell banking
Neuroglia - cytology
Neurons - cytology
Postmortem Changes
Prosencephalon - cytology
Spinal Cord - cytology
Stem Cells - cytology
Temperature
Time Factors
tissue culture
neural stem cell banking
neural stem and progenitor cells
extended postmortem intervals
tissue culture
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Summary:The adult mammalian CNS harbors a population of multipotent stem/progenitor cells that can be induced to grow as proliferative neurospheresin vitro.We demonstrate here that neurosphere-generating cells can be isolated from adult mouse spinal cord and forebrain subependymal zone after postmortem intervals of up to 140 h, when kept at 4°C, and up to 30 h when kept at room temperature. Although there was an inverse relationship between postmortem interval and the number of neurospheres generated, neurospheres derived under these conditions were proliferative and could give rise to both neurons and glia.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4886
1090-2430
DOI:10.1006/exnr.1999.7029