In-vitro evaluation of MPA-loaded electrospun coaxial fiber membranes for local treatment of glioblastoma tumor cells

• Published in: Journal of drug delivery science and technology Vol. 40; pp. 45 - 50
• Main Authors: Han, Daewoo, Sasaki, Mika, Yoshino, Hirofumi, Kofuji, Satoshi, Sasaki, Atsuo T., Steckl, Andrew J.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2017
• Subjects: Brain cancer; Coaxial electrospinning; Drug delivery; Glioblastoma; Mycophenolic acid; Nanofiber; Nanofiber; Drug delivery; Mycophenolic acid; Coaxial electrospinning; Brain cancer; Glioblastoma
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2017.05.017

Core-sheath fibers containing a drug for brain tumor are reported. Mycophenolic acid (MPA), a FDA-approved immunosuppressant, has been demonstrated to inhibit several types of tumor cells growth. However, the effective serum MPA concentration for anti-tumor declines quickly in-vivo due to degradation in the liver, which hampers the development of MPA-based anti-tumor therapy. To overcome this issue, we have formed MPA-containing electrospun fiber membranes as local drug delivery vehicle and characterized MPA release profiles based on fiber composition and geometry. Coaxial fibers with poly(ε-caprolactone) (PCL)/MPA core and PCL sheath provided a more sustained release than homogenous fibers. In particular, thicker PCL sheath with 1:10 ratio of sheath thickness to fiber diameter provides gradual release in an initial period and higher MPA release after refreshing of media. The host polymer for MPA has a significant effect on the MPA release, with PCL/MPA single fiber providing more sustained release than coaxial fibers with polyvinylpyrrolidone (PVP)/MPA core and PCL sheath. In-vitro glioblastoma multiforme (GBM) tumor cell culture results show strong cell suppression effect by MPA-containing fiber membranes, with coaxial fiber membranes inhibiting GBM cell growth 3-5 × more than the single fiber membranes. This indicates that MPA-containing electrospun membranes have a promising potential for local treatment of GBM. [Display omitted]