Tagging of four fertility restorer loci for wild abortive--cytoplasmic male sterility system in rice (Oryza sativa L.) using microsatellite markers

• Published in: Euphytica Vol. 164; no. 3; pp. 669 - 677
• Main Authors: Bazrkar, L, Ali, A. J, Babaeian, N. A, Ebadi, A. A, Allahgholipour, M, Kazemitabar, K, Nematzadeh, G
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.12.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agricultural biotechnology; Agronomy. Soil science and plant productions; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; chromosome mapping; Chromosomes; Classical and quantitative genetics. Population genetics. Molecular genetics; Crop science; cytoplasmic male sterility; Fertility; Fundamental and applied biological sciences. Psychology; Gene loci; Generalities. Genetics. Plant material; Genetic markers; genetic recombination; Genetically engineered microorganisms; Genetics and breeding of economic plants; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia; Life Sciences; loci; Male sterility; microsatellite repeats; Molecular genetics; Oryza sativa; Plant breeding; Plant breeding: fundamental aspects and methodology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; random mating composite population; restorer lines; Rf genes; Rf loci; Rice; Risk assessment; wild abortive cytoplasmic male sterility; Tagging; Fertility restorer; Microsatellite markers; CMS-WA; Monocotyledones; Restoration of fertility; Molecular marker; Cereal crop; Oryza sativa; Gramineae; Microsatellite DNA; Angiospermae; Genetic improvement; Spermatophyta; Cytoplasmic male sterility
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-008-9667-8

Tagging of restorer genes for wild abortive (WA) CMS source by studying a 222 individual plants from a F₂ population of a cross between IR58025A x IR42686R. The restorer line IR42686R that was used in this study had been previously derived through random mating composite population (RMCP) involving 12 parents facilitated by IR36 genetic male sterility. Four Rf genes were tagged to simple sequence repeats (SSR) markers on chromosomes 1, 7, 10, 12 by recessive class analysis. The recombination frequency between a positive marker and Rf locus was calculated using maximum likelihood estimator assuming that all the 46 extremely sterile individual plants were homozygous at the targeted Rf locus. The recombination frequency between the marker and the restorer trait were converted to genetic distances using Kosambi function. A new Rf locus designated as Rf7 on chromosome 12 was found to be linked to RM7003 at a genetic distance of 13.3 cM (LOD 6.12). We report here first, a new molecular marker (RM 6344) linked to Rf4 locus on chromosome 7 that was previously mapped by trisomic analysis. RM443 and RM315 were flanking the Rf3 gene at a genetic distance of 4.4 (LOD 10.29) and 20.7 cM (LOD 3.98) on chromosome 1, respectively. The Rf6 was flanked on both side with SSR markers RM258 and RM591 at a genetic distance of 4.4 (LOD 10.29) and 23.3 cM (LOD 3.39) located on chromosome 10. The random mating composite population is an excellent breeding approach to develop superior restorer lines and for pyramiding different Rf genes of different CMS systems. Rf genes tagged with closely linked SSR markers would be facilitating marker assisted selection (MAS) in hybrid rice breeding program by reducing time and workload for identifying potential restorers.