The influence of nitrapyrin and sodium azide on the growth and respiration of some Rhizobium strains

• Published in: Microbiological research Vol. 151; no. 3; pp. 247 - 251
• Main Authors: Bayoumi Hamuda, H.E.A.F., Kiss, Zita, Várady, György, Kucsma, Nóra, Balázsy, Sándor, Kecskés, Mihály
• Format: Journal Article
• Language: English
• Published: Basingstoke Elsevier GmbH 1996; Nature Publishing Group
• Subjects: Action of physical and chemical agents on bacteria; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microbiology; Nitrapyrin; respiration; Rhizobium; Rhizobium leguminosarum; Rhizobium trifolii; sodium azide; survival; Rhizobium; respiration; sodium azide; survival; Nitrapyrin; Microorganism growth; Growth rate; Nitrification inhibitor; In vitro; Survival; Biological activity; Pyridine derivatives; Sodium Azides; Bacteria; Rhizobiaceae; Antibacterial agent; Cell respiration
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/S0944-5013(96)80020-6

In vitro addition of different concentrations of nitrification inhibitor nitrapyrin and respiratory inhibitor sodium azide to the growth media of seven Rhizobium strains representing the four Rhizobium species ( R. leguminosarum, R. phaseoli, R. trifolii and R. loti) influenced the growth of these strains within 48 h at 28 °C incubation on a rotary shaker. All doses of nitrapyrin inhibited growth. In contrast, 0.1 mg 1 -1 enhanced the respiration rate of the strains using Warburg's respirometer. The doses 10 and 100 mg 1 -1 were highly toxic and depressed the respiration rates of Rhizobium within 180 minutes. Application of 10 mM NaN 3 enhanced the growth and respiration rates of all the strains. The growth of R. leguminosarum (Lóbab Z and Bükköny 75/4 strains) was stimulated by 20 mM and also R. phaseoli (Bab 5/3 strain) by 30 mM NaN 3. The growth and respiration rates of the tested Rhizobium strains were significantly depressed by 50 mM NaN 3, whereas the respiration rates of the strains Lóbab Z, Bükköny 75/4 and Bab 5/3 at 30 mM NaN 3 were higher than the control. However, 50 mM NaN 3 was significantly high and depressed the respiration rates of Rhizobium strains at P = 0.05.