Expression of the histone chaperone SET/TAF-Iβ during the strobilation process of Mesocestoides corti (Platyhelminthes, Cestoda)

• Published in: Parasitology Vol. 142; no. 9; pp. 1171 - 1182
• Main Authors: COSTA, CAROLINE B., MONTEIRO, KARINA M., TEICHMANN, ALINE, DA SILVA, EDILEUZA D., LORENZATTO, KARINA R., CANCELA, MARTÍN, PAES, JÉSSICA A., BENITZ, ANDRÉ DE N. D., CASTILLO, ESTELA, MARGIS, ROGÉRIO, ZAHA, ARNALDO, FERREIRA, HENRIQUE B.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.08.2015
• Subjects: Amino Acid Sequence; Animals; Cestode Infections - parasitology; Cloning, Molecular; DNA, Complementary - genetics; DNA, Complementary - metabolism; Female; Gene Expression Regulation - physiology; Helminth Proteins - genetics; Helminth Proteins - metabolism; Histone Chaperones - genetics; Histone Chaperones - metabolism; Mesocestoides - metabolism; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; strobilation process; Mesocestoides corti; cestode development; SET/TAF-Iβ
• ISSN: 0031-1820; 1469-8161; 1469-8161
• DOI: 10.1017/S003118201500030X

The histone chaperone SET/TAF-Iβ is implicated in processes of chromatin remodelling and gene expression regulation. It has been associated with the control of developmental processes, but little is known about its function in helminth parasites. In Mesocestoides corti, a partial cDNA sequence related to SET/TAF-Iβ was isolated in a screening for genes differentially expressed in larvae (tetrathyridia) and adult worms. Here, the full-length coding sequence of the M. corti SET/TAF-Iβ gene was analysed and the encoded protein (McSET/TAF) was compared with orthologous sequences, showing that McSET/TAF can be regarded as a SET/TAF-Iβ family member, with a typical nucleosome-assembly protein (NAP) domain and an acidic tail. The expression patterns of the McSET/TAF gene and protein were investigated during the strobilation process by RT-qPCR, using a set of five reference genes, and by immunoblot and immunofluorescence, using monospecific polyclonal antibodies. A gradual increase in McSET/TAF transcripts and McSET/TAF protein was observed upon development induction by trypsin, demonstrating McSET/TAF differential expression during strobilation. These results provided the first evidence for the involvement of a protein from the NAP family of epigenetic effectors in the regulation of cestode development.