Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum
Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS 2 ) analysis under thoroughly optimized experi...
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| Published in | Analytical letters Vol. 44; no. 6; pp. 1036 - 1049 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Philadelphia, PA
Taylor & Francis Group
29.03.2011
Taylor & Francis |
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| Abstract | Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS
2
) analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS
2
molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range. |
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| AbstractList | Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS
2
) analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS
2
molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range. Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS2) analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS2 molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range. |
| Author | Vukelić, Željka Ghiulai, Roxana M. Zamfir, Alina D. Mosoarca, Cristina Chiriac, Adrian Novaconi, Cristina R. |
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| Keywords | Cerebellum Ion trap mass spectrometry Electrospray Mixture Ion trap Efficiency High speed Ion spectrometry Dissociation Chip-nanoelectrospray Human Automation Collisional activation Sample System on a chip Method Molecular ions Ganglioside NanoMate Ionization Reproducibility Mass spectrometry MS/MS Application Mass spectrometry Gangliosides Structural analysis |
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| SubjectTerms | Analytical chemistry Automation Cerebellum Chemistry Chip-nanoelectrospray Exact sciences and technology Gangliosides Ion trap mass spectrometry NanoMate Spectrometric and optical methods |
| Title | Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum |
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