Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum

• Published in: Analytical letters Vol. 44; no. 6; pp. 1036 - 1049
• Main Authors: Mosoarca, Cristina, Ghiulai, Roxana M., Novaconi, Cristina R., Vukelić, Željka, Chiriac, Adrian, Zamfir, Alina D.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 29.03.2011; Taylor & Francis
• Subjects: Analytical chemistry; Automation; Cerebellum; Chemistry; Chip-nanoelectrospray; Exact sciences and technology; Gangliosides; Ion trap mass spectrometry; NanoMate; Spectrometric and optical methods; Cerebellum; Ion trap mass spectrometry; Electrospray; Mixture; Ion trap; Efficiency; High speed; Ion spectrometry; Dissociation; Chip-nanoelectrospray; Human; Automation; Collisional activation; Sample; System on a chip; Method; Molecular ions; Ganglioside; NanoMate; Ionization; Reproducibility; Mass spectrometry MS/MS; Application; Mass spectrometry; Gangliosides; Structural analysis
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032719.2010.506938

Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS 2 ) analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS 2 molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range.