Antiviral Effects of Aqueous Extract from Spatholobus suberectus Dunn. against Coxsackievirus B3 in Mice

• Published in: Chinese journal of integrative medicine Vol. 17; no. 10; pp. 764 - 769
• Main Author: 庞佶 郭金鹏 金敏 谌志强 王新为 李君文
• Format: Journal Article
• Language: English
• Published: Heidelberg Chinese Association of Traditional and Western Medicine 01.10.2011
• Subjects: Animals; Antiviral Agents - pharmacology; Antiviral Agents - therapeutic use; Body Weight - drug effects; Cercopithecus aethiops; Coxsackievirus Infections - blood; Coxsackievirus Infections - drug therapy; Coxsackievirus Infections - pathology; Coxsackievirus Infections - virology; Enterovirus - drug effects; Fabaceae - chemistry; Gene Expression Regulation - drug effects; Male; Medicine; Medicine & Public Health; Mice; Mice, Inbred BALB C; mRNA表达; Myocardium - pathology; Organ Size - drug effects; Original Article; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Survival Analysis; Vero Cells; Viral Load; 体外实验; 小鼠; 抗病毒效果; 柯萨奇B3病毒; 柯萨奇病毒; 水提取物; 鸡血藤; Dunn; coxsackievirus B3; viral myocarditis; aqueous extract of
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-011-0642-1

Objective： To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. （A.E.）, a Chinese medicinal herb, against coxsackievirus B3 （CVB3）. Methods： The antiviral effects of A.E. against CVB3 in vitro （primarily cultured myocardial cells） and in vivo （BALB/c mice） were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo. Results： A.E. exhibited obvious antiviral effects in vivo, and serum samples obtained from the rats with oral administration of A.E. （10 μg/mL, 5 μg/mL） reduced the virus titers in the infected myocardial cells （3.00±0.70, 3.55±0.52, P〈0.01）. Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% （P〈0.01） in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively （P〈0.01）. Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E. Conclusion： Aqueous extract of Spatho/obus suberectus Dunn. （A.E.） has inhibitory effect on CVB3 both in vitro and in vivo.