Temporal Dynamics of Fungal Communities in Alkali-Treated Round Bamboo Deterioration under Natural Weathering

• Published in: Microorganisms (Basel) Vol. 12; no. 5; p. 858
• Main Authors: Han, Shuaibo, An, Xiaojiao, He, Xiaolong, Ren, Xin, Sichone, John, Wu, Xinxing, Zhang, Yan, Wang, Hui, Sun, Fangli
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.04.2024
• Subjects: alkali treatment; Alkalies; Ascomycota; Bamboo; bamboo deterioration; Basidiomycota; Cell walls; Cellulose; Contact angle; Deterioration; Dynamic structural analysis; Environmental aspects; Environmental factors; Environmental protection; Epidermis; fungal community; Fungi; Hemicellulose; Humidity; Lignin; Methods; Microorganisms; Moisture content; Mold; Next-generation sequencing; Physiological aspects; Relative abundance; Spectrum analysis; Weathering; China; fungal community; bamboo deterioration; alkali treatment
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12050858

Microbes naturally inhabit bamboo-based materials in outdoor environments, sequentially contributing to their deterioration. Fungi play a significant role in deterioration, especially in environments with abundant water and favorable temperatures. Alkali treatment is often employed in the pretreatment of round bamboo to change its natural elastic and aesthetic behaviors. However, little research has investigated the structure and dynamics of fungal communities on alkali-treated round bamboo during natural deterioration. In this work, high-throughput sequencing and multiple characterization methods were used to disclose the fungal community succession and characteristic alterations of alkali-treated round bamboo in both roofed and unroofed habitats throughout a 13-week deterioration period. In total, 192 fungal amplicon sequence variants (ASVs) from six phyla were identified. The fungal community richness of roofed bamboo samples declined, whereas that of unroofed bamboo samples increased during deterioration. The phyla Ascomycota and Basidiomycota exhibited dominance during the entire deterioration process in two distinct environments, and the relative abundance of them combined was more than 99%. A distinct shift in fungal communities from Basidiomycota dominant in the early stage to Ascomycota dominant in the late stage was observed, which may be attributed to the increase of moisture and temperature during succession and the effect of alkali treatment. Among all environmental factors, temperature contributed most to the variation in the fungal community. The surface of round bamboo underwent continuous destruction from fungi and environmental factors. The total amount of cell wall components in bamboo epidermis in both roofed and unroofed conditions presented a descending trend. The content of hemicellulose declined sharply by 8.3% and 11.1% under roofed and unroofed environments after 9 weeks of deterioration. In addition, the contact angle was reduced throughout the deterioration process in both roofed and unroofed samples, which might be attributed to wax layer removal and lignin degradation. This study provides theoretical support for the protection of round bamboo under natural weathering.