Evaluation of a modified reverse line blot assay for detection and typing of human papillomavirus

• Published in: American journal of clinical pathology Vol. 123; no. 6; pp. 896 - 899
• Main Authors: TABRIZI, Sepehr N, STEVENS, Matthew, SHUJUN CHEN, RUDLAND, Elice, KORNEGAY, Janet R, GARLAND, Suzanne M
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Society of Clinical Pathologists 01.06.2005
• Subjects: Biological and medical sciences; Female; Humans; In Situ Hybridization - methods; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Nucleic Acid Amplification Techniques; Papillomaviridae - isolation & purification; Papillomavirus Infections - diagnosis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Reproducibility of Results; Sensitivity and Specificity; Uterine Cervical Neoplasms - virology; Papillomavirus; Virus; Evaluation; Anatomic pathology; Human papillomavirus; Typing; Papovaviridae; Diagnosis; Detection
• ISSN: 0002-9173; 1943-7722
• DOI: 10.1309/MPERNJ0G62RECHCQ

Detection and typing of human papillomaviruses (HPVs) are requested by clinicians with growing frequency as part of the overall management of patients. A new method using consensus L1 amplification and a reverse line blot hybridization detection method has been described for broad spectrum HPV genotype identification. This method involves hybridization at 53 degrees C in a shaking water bath, which many laboratories might find cumbersome. We describe a modified hybridization step, using a dry-air incubator, that simplifies the detection protocol. Overall, comparable results were obtained by the modified, more easily performed protocol, indicating that laboratories might validate and use this modified method.