Bioelectrochemistry of microperoxidases

• Published in: Bioelectrochemistry and Bioenergetics Vol. 28; no. 1; pp. 159 - 176
• Main Authors: Razumas, V., Kazlauskaite, J., Ruzgas, T., Kulys, J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier S.A 1992; Elsevier Science
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Cytochrome c; Hemoprotein; Vertebrata; Mammalia; Enzyme; Peroxidase; Structure function relationship
• ISSN: 0302-4598
• DOI: 10.1016/0302-4598(92)80010-E

This paper reports on an experimental study of the structure and functioning of heme-containing polypeptides (microperoxidases-8, -9 and -11) at the solid-electrolyte interface. The electrochemical and electrocatalytical properties of microperoxidases were investigated by electrochemical methods applying different electrode materials, whereas the adsorption and structure of these polypeptides were studied ellipsometrically and using surface-enhanced Raman spectroscopy. From the results obtained it was found that microperoxidases are useful models for investigating the interfacial behaviour of heme-containing proteins. The low molecular weight of the peptide chain and high exposure of the heme groups are the principal determinants for the observed direct electrochemical reactions. The influence of l-histidine coordination, the amino acid sequence of the polypeptide chain and surface hydrophobic-hydrophilic properties on the electrochemical and interfacial behaviour of microperoxidases were investigated.