Analysis of fragments of the rpb1 gene sequences of diatoms

• Published in: Doklady. Biochemistry and biophysics Vol. 422; no. 1; pp. 285 - 291
• Main Authors: Gabaev, I. I., Likhoshvai, E. V., Shcherbakova, T. A., Ostyak, A. S.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.10.2008; Springer Nature B.V
• Subjects: Algae; Amino acids; Base Sequence; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Diatoms - genetics; Eukaryotes; Evolution, Molecular; Genetic markers; Life Sciences; Molecular Biology; Molecular Sequence Data; Polymorphism; Protein Subunits; Ribonucleic acid; RNA; RNA Polymerase II - genetics; Sequence Analysis, DNA; Rpb1 Sequence; Relevant Fragment; DOKLADY Biochemistry; Rpb1 Gene; Phaeodactylum Tricornutum
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672908050098

A study determined the sequences of the rpb1 gene fragment in diatoms of different taxonomic level; to perform a comparative analysis of these sequences with the homologous sequences of protists, fungi, plants, animals, and archaea deposited in GenBank; and to assess the applicability of the rpb1 fragment for use as a phylogenetic marker. This was the first study to decode the sequences of the nuclear gene rpb1 of several diatom species from different classes. The sufficiently high level of variability of the relevant fragment in diatoms makes it possible to perform genetic reconstructions with resolution at the generic level.