Molecular cloning and expression of a Bacillus subtilis beta-glucanase gene in Escherichia coli

A Bacillus subtilis gene coding for an endo-beta-1,3-1,4-glucanase has been transferred to Escherichia coli by molecular cloning using bacteriophage lambda and plasmid vectors. The gene is contained within a 1.6-kb EcoRI-PvuI DNA fragment and directs the synthesis in E. coli of a beta-glucanase whic...

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Bibliographic Details
Published inGene Vol. 23; no. 2; pp. 211 - 219
Main Authors Cantwell, B A, McConnell, D J
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1983
Subjects
Bacillus subtilis
Bacillus subtilis - genetics
Bacteriophage lambda - genetics
Chromosome Mapping
Cloning, Molecular
DNA Restriction Enzymes
Escherichia coli - genetics
Genes
Genes, Bacterial
Genetic Techniques
Glycoside Hydrolases - genetics
Plasmids
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Summary:A Bacillus subtilis gene coding for an endo-beta-1,3-1,4-glucanase has been transferred to Escherichia coli by molecular cloning using bacteriophage lambda and plasmid vectors. The gene is contained within a 1.6-kb EcoRI-PvuI DNA fragment and directs the synthesis in E. coli of a beta-glucanase which specifically degrades barley glucan and lichenan. A novel dye-staining method has been developed to detect beta-glucanase activity in colonies on agar plates.
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ISSN:0378-1119
DOI:10.1016/0378-1119(83)90053-7