Association of Artery Wall Hypoxia and Cellular Proliferation at a Vascular Anastomosis

• Published in: The Journal of surgical research Vol. 91; no. 1; pp. 32 - 37
• Main Authors: Lee, Eugene S., Bauer, G.Eric, Caldwell, Michael P., Santilli, Steven M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Elsevier Inc 01.06.2000; Elsevier
• Subjects: Anastomosis, Surgical; Animals; Aorta, Abdominal - chemistry; Aorta, Abdominal - pathology; Aorta, Abdominal - surgery; artery; Biological and medical sciences; Blood and lymphatic vessels; Blood Vessel Prosthesis Implantation; Bromodeoxyuridine - analysis; Cardiology. Vascular system; Cell Division - physiology; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Hyperplasia; hypoxia; Hypoxia - pathology; intimal hyperplasia; Medical sciences; Muscle, Smooth, Vascular - chemistry; Muscle, Smooth, Vascular - pathology; Muscle, Smooth, Vascular - surgery; Oxygen Consumption; Polytetrafluoroethylene; Rabbits; smooth muscle cells; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tunica Intima - chemistry; Tunica Intima - pathology; Tunica Intima - surgery; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels; smooth muscle cells; hypoxia; intimal hyperplasia; artery; Oxygen; Wall; Hyperplasia; Rate; Surgical anastomosis; Rabbit; Cardiovascular disease; Lagomorpha; Experimental study; Artery; Vascular disease; Vertebrata; Mammalia; Treatment; Surgery; Animal; Intima; Hypoxia; Biological effect
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1006/jsre.2000.5891

Background. We hypothesize that arterial wall hypoxia incites the pathologic formation of intimal hyperplasia at an artery anastomosis. We have determined from previous studies performed in our laboratory, the oxygen tension profiles of the artery wall at various times after vascular anastomosis. The purpose of this study is to determine the rate of cellular proliferation at an artery anastomosis when the artery wall is most hypoxic. Materials and methods. Expanded polytetrafluoroethylene (ePTFE) grafts were placed end to end in the infrarenal aorta of 27 New Zealand white rabbits. The anastomotic aortic wall oxygen (O2) tensions were measured with an O2 microelectrode in rabbits 0, 7, 14, 28, and 42 days after surgery. O2 tensions were also measured in 4 control rabbits for comparison. 5-Bromo-2′-deoxyuridine (BrDU) was injected intraperitoneally 24 h prior to rabbit sacrifice. After O2 tension measurements, the rabbits were sacrificed and the aortic grafts were harvested. Bioquant morphometrics was used to measure cells with BrDU counterstaining and intimal thickness in 17 rabbits: in control (n = 4), Day 0 (n = 4), 7 (n = 5), and 42 (n = 4). Student's t test was used to compare O2 tensions, cellular proliferation, and intimal hyperplasia between days. Results. The pO2 levels at the outer layers of the aorta, 1 mm distal to the distal aortic graft anastomosis, were 61.0 ± 2 (±SE) mm Hg for controls, 19.8 ± 1 mm Hg for Day 7 (P < 0.0001), 19.0 ± 1 mm Hg for Day 14, 39.2 ± 1 mm Hg for Day 28, and 58.5 ± 1 mm Hg for Day 42 aortic grafts. BrDU-staining ratios in the intima were significantly higher in the Day 7 aortic grafts, 28.6 ± 3%, versus BrDU-staining ratio, 1.4 ± 1%, in Day 42 aortic grafts (P < 0.0002). Conclusions. Cellular proliferation is highest at Day 7 when the artery wall is most hypoxic and returns to baseline as O2 tensions normalize.