RFLP tagging of a new semidwarfing gene in rice

A new rice semidwarfing gene which is not allelic to sd1, temporarily designated as sdg, might be of use as a new source of semidwarfism in rice breeding programs. The identification of a DNA marker closely linked to this gene is reported. The DNA marker was identified by testing 120 mapped rice RFL...

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Published inTheoretical and applied genetics Vol. 88; no. 6-7; p. 898
Main Authors Liang, C.Z, Gu, M.H, Pan, X.B, Liang, G.H, Zhu, L.H. (Academia Sinica, Beijing (China). Inst. of Genetics)
Format Journal Article
LanguageEnglish
Published Germany 01.08.1994
Subjects
ADN
ENANISMO
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
GENE
GENES
MARCADORES GENETICOS
MARQUEUR GENETIQUE
NANISME
ORYZA SATIVA
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
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Summary:A new rice semidwarfing gene which is not allelic to sd1, temporarily designated as sdg, might be of use as a new source of semidwarfism in rice breeding programs. The identification of a DNA marker closely linked to this gene is reported. The DNA marker was identified by testing 120 mapped rice RFLP makers as hybridization probes for Southern analysis of a pair of nearly isogenic lines with or without sdg. Linkage association of the marker with the gene was verified using a F2 population segregating for semidwarfism. RFLP analysis showed that sdg is closely linked to a single-copy DNA clone RZ182 on chromosome 5, with a distance of 4.3 centiMorgans between them. This marker may facilitate early selection for the semidwarfing gene in rice breeding programs
Bibliography:97B7055
F30
ISSN:0040-5752
1432-2242
DOI:10.1007/BF01254003