Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans

• Published in: Journal of microbiology and biotechnology Vol. 28; no. 6; pp. 883 - 892
• Main Authors: Sim, Insuk, Park, Keun-Tae, Kwon, Gayeung, Koh, Jong-Ho, Lim, Young-Hee
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.06.2018
• Subjects: 생물학; Caenorhabditis elegans; Enterococcus faecium; immunomodulatory activity; lifespan extension; probiotics
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1802.02019

Probiotics including confer a health benefit on the host. An strain was isolated from healthy chicken cecum, identified as 16S rDNA gene sequence analysis, and designatedas L11.Toevaluate the potential of L11 as a probiotics, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. L11 showed >66% and >62% survival in artificial gastric juices (0.3% pepsin, pH 2.5) and simulated smallintestinal juice(0.5% bile salt and 0.1%pancreatin), respectively. Heat-killed L11significantly ( < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines(IL-6 and TNF-α) by activated macrophages obtained from ICRmice. In addition, L11 showed a protective effect against Typhimurium infection in . In addition, feeding L11 significantly ( < 0.05) extended the lifespan of compared with the control. Furthermore, genes related to aging and host defense were upregulated in L11-fed worms. In conclusion, L11 which prolongs the lifespan of may be a potent probiotic supplement for livestock.