Incorporating Antimicrobial Nanomaterial and its Effect on the Antimicrobial Activity, Flow and Radiopacity of Endodontic Sealers

• Published in: European endodontic journal Vol. 2; no. 1; p. 1
• Main Authors: Teixeira, Ana Beatriz Vilela, Vidal, Carla Larissa, de Castro, Denise Tornavoi, de Oliveira-Santos, Christiano, Schiavon, Marco Antônio, Dos Reis, Andréa Cândido
• Format: Journal Article
• Language: English
• Published: Turkey 2017
• Subjects: endodontics; nanotechnology; Antimicrobial agents; radiography dental digital
• ISSN: 2548-0839; 2548-0839
• DOI: 10.5152/eej.2017.16029

This preliminary study aimed to evaluate the antimicrobial activity, flow and radiopacity of endodontic sealers with nanostructured silver vanadate decorated with silver nanoparticles (AgVO3). The minimum inhibitory concentration (MIC) of AgVO3 was evaluated against . Specimens were prepared from the following endodontic sealers: AH Plus (DENTSPLY DeTrey GmbH, Konstanz, Germany), Sealapex (Sybron Endo, Orange, CA, USA), Sealer 26 (DENTSPLY, Petrópolis, Brazil) and Endofill (DENTSPLY, Petrópolis, Brazil), with concentrations of 0%, 2.5%, 5% and 10% of AgVO3. Agar diffusion was used to evaluate the materials after 48 hours and 7 days (n=6). Flow (n=6) and radiopacity (n=9) were evaluated. The data were analysed by analysis of variance (ANOVA) and the Tukey honestly significant difference (HSD) (α=0.05). The MIC of AgVO3 was 500 μg/mL for and 31.25 μg/mL for and . The AgVO3 did not influence the antimicrobial activity of AH Plus against (P>0.05) but did promote this activity for Sealapex (P<0.01). Moreover, this activity increased for Endofill from 2.5% and for Sealer 26 from 5% (P<0.05). Against , only AH Plus and Endofill 10% inhibited zone formation (P<0.01). The antimicrobial activity of Endofill increased from 2.5% against (P<0.01). Sealapex 5% and 10% (P<0.01), Sealer 26 10% and AH Plus promoted antimicrobial activity against E. coli. An increase in the zone of inhibition occurred between 48 hours and 7 days in the Sealapex 10% and Endofill 5% groups against E. coli. The flow of AH Plus and Endofill decreased with the increase of AgVO3 (P<0.05), and the flow of Sealer 26 and Sealapex was not affected (P>0.05). The radiopacity of AH Plus increased with AgVO3 (P<0.05). Endofill 5% and 10% did not differ from the control Endofill (P>0.05). The incorporation of AgVO3 did not influence the radiopacity of Sealer 26 (P>0.05). The incorporation of 2.5% and 5% AgVO3 reduced the radiopacity of Sealapex (P<0.05). Adding AgVO3 may increase the antimicrobial effect of endodontic sealers without major changes in their physicochemical properties.