Production of monoclonal antibodies against Clostridium difficile cytotoxin using immunosorbent binding bioassay procedure

In the following study, a novel screening approach was used to develop monoclonal antibodies specific for toxin B of Clostridium difficile. The approach, which consisted of an immunosorbent binding bioassay (ISBBA), is based on antigen immunocapture by monoclonal antibodies and detection of biologic...

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Bibliographic Details
Published inToxicon (Oxford) Vol. 31; no. 2; p. 181
Main Authors Depitre, C, L'Haridon, R M, Lyerly, D M, Barroso, L A, Wilkins, T D, Andremont, A, Corthier, G
Format Journal Article
LanguageEnglish
Published England 01.02.1993
Subjects
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - biosynthesis
Ascites - immunology
Bacterial Proteins
Bacterial Toxins - immunology
Blotting, Northern
Clostridium difficile - metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Hybridomas - immunology
Immunosorbent Techniques
Mice
Neutralization Tests
Rats
Spleen - cytology
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Summary:In the following study, a novel screening approach was used to develop monoclonal antibodies specific for toxin B of Clostridium difficile. The approach, which consisted of an immunosorbent binding bioassay (ISBBA), is based on antigen immunocapture by monoclonal antibodies and detection of biological activity. Our results showed ISBBA, which uses unpurified antigen, to be more sensitive than the neutralization assay and ELISA for the detection of toxin B antibody.
ISSN:0041-0101
DOI:10.1016/0041-0101(93)90284-P