Mismatch binding ligand upregulated back-splicing reaction producing circular RNA in a cellular model

Circular RNA (circRNA) is a covalently closed single-stranded RNA produced from pre-mRNAs via back-splicing reaction, an alternative form of splicing. Here, we show naphthyridine carbamate dimer (NCD) upregulating the production of a circRNA from a pre-mRNA containing NCD-binding site UGGAA/UGGAA in...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 58; no. 22; pp. 3629 - 3632
Main Authors Ni, Lu, Yamada, Takeshi, Murata, Asako, Nakatani, Kazuhiko
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 15.03.2022
Subjects
Binding sites
Dimers
Ligands
RNA - genetics
RNA - metabolism
RNA Precursors - genetics
RNA Splicing
RNA, Circular
Splicing
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Summary:Circular RNA (circRNA) is a covalently closed single-stranded RNA produced from pre-mRNAs via back-splicing reaction, an alternative form of splicing. Here, we show naphthyridine carbamate dimer (NCD) upregulating the production of a circRNA from a pre-mRNA containing NCD-binding site UGGAA/UGGAA in cells, demonstrating the feasibility of small-molecule mediated circRNA production. Circular RNAs (circRNA) are covalently-closed single stranded RNAs with potent roles in cells. Here, we demonstrate the feasibility of mediating circRNA production in a model environment via a small molecule: naphthyridine carbamate dimer (NCD).
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/d1cc06936e
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc06936e