Isolation and characterization of a novel anionic peroxidase cDNA found in poplar (Populus nigra) suspension cultured cells

• Published in: Journal of wood science Vol. 47; no. 3; pp. 165 - 170
• Main Authors: Kato, Y. (Iwate-ken. Biotechnology Research Center, Kitakami (Japan)), Sakuma, Y, Azuma, T, Ando, A, Miura, K, Takabe, K, Tamai, Y
• Format: Journal Article
• Language: English
• Published: Tokyo Springer 01.06.2001; Springer Nature B.V
• Subjects: BARK; Biological and medical sciences; CELL CULTURE; DNA; Fundamental and applied biological sciences. Psychology; Gene expression; GENETIC CODE; Peroxidase; PEROXIDASES; Plant growth. Development of the storage organs; Plant physiology and development; Poplar; POPULUS NIGRA; Proteins; Stems; Vegetative apparatus, growth and morphogenesis. Senescence; Nucleotide sequence; Enzyme; Populus nigra; Bark; Tissue specificity; Homology; Gene expression; Salicaceae; Messenger RNA; Peroxidases; Complementary DNA; Dicotyledones; Angiospermae; Development; Spermatophyta; Hardwood forest tree; Peroxidase; Oxidoreductases; Molecular cloning; Aminoacid sequence
• ISSN: 1435-0211; 1611-4663
• DOI: 10.1007/BF01171217

A cDNA clone from Populus nigra L. var, italica Koehne, denoted PCY2-6, coding for an anionic peroxidase has been isolated, cloned, and characterized. PCY2-6 is 1160bp long; and its deduced product, PnC26, contains 343 amino acid residues. The mature protein has a calculated isoelectric point of 4.09. The protein contains two motifs typical of peroxidase and 10 potential N-glycosylation sites. PnC26 is therefore classified as an anionic peroxidase. The mRNA of the PCY2-6 gene family was detected in immature and mature leaves and in two parts of current-year stems: the shoot tip and the older stem. The mRNA of PCY2-6 gene family was found to localize in the phloem and cortex of the current-year stems. We therefore conclude that expression of the PCY2-6 gene family is related to bark development.