Effects of cerous nitrate on growth and tanshinone production in salvia miltiorrhiza hairy roots

Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce^3＋ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The r...

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Published inJournal of rare earths Vol. 33; no. 11; pp. 1228 - 1235
Main Author 韩名宇 郭万里 梁宗锁 杨东风 闫希军 朱永宏 刘岩
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.11.2015
Subjects
Biosynthesis
Ce3
Genes
Medical services
rare earth element
Rare earth metals
Roots
Salvia miltiorrhiza
secondary metabolism
Tanshinones
Traditional chinese medicine
丹参毛状根
丹参酮ⅡA
根生长
活性成分
生产
生物合成机制
硝酸铈
隐丹参酮
Ce3
rare earth element
tanshinones
Salvia miltiorrhiza
secondary metabolism
Online AccessGet full text
ISSN1002-0721
2509-4963
DOI10.1016/S1002-0721(14)60549-4

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Summary:Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce^3＋ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce^3＋, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce^3＋. Responses of DXS1, DXS2 and GGPPS to Ce^3＋ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
Bibliography:tanshinones Salvia miltiorrhiza Ce^3＋ secondary metabolism rare earth element
11-2788/TF
Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce^3＋ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce^3＋, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce^3＋. Responses of DXS1, DXS2 and GGPPS to Ce^3＋ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
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ISSN:1002-0721
2509-4963
DOI:10.1016/S1002-0721(14)60549-4