Measurement of feruloyl/p-coumaroyl esterase by capillary zone electrophoresis

Ferulic andp-coumaric acid can be separated from their corresponding aliphatic methyl esters by capillary zone electrophoresis, which allows the convenient determination of feruloyl andp-coumaroyl esterase activities using synthetic esters as substrates. A feruloyl-containing sugar ester from wheat...

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Bibliographic Details
Published inWorld journal of microbiology & biotechnology Vol. 11; no. 2; pp. 160 - 162
Main Authors Donaghy, J.A, McKay, A.M
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.03.1995
Subjects
aliphatic methyl esters
Biological and medical sciences
Biotechnology
electrophoresis
enzyme activity
Enzyme engineering
esterases
esters
ferulic acid
feruloyl esterase
Fundamental and applied biological sciences. Psychology
measurement
Methods. Procedures. Technologies
Miscellaneous
p-coumaric acid
Penicillium expansum
wheat bran
Monocotyledones
Enzyme
Gel electrophoresis
Esterases
Capillary tube
Fungi
Penicillium expansum
Enzymatic activity
Gramineae
Angiospermae
Hydrolases
Spermatophyta
Fungi Imperfecti
Triticum aestivum
Thallophyta
Quantitative analysis
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Summary:Ferulic andp-coumaric acid can be separated from their corresponding aliphatic methyl esters by capillary zone electrophoresis, which allows the convenient determination of feruloyl andp-coumaroyl esterase activities using synthetic esters as substrates. A feruloyl-containing sugar ester from wheat bran was also efficiently separated and used as substrate for the enzyme assays.Penicillium expansum was shown to produce feruloyl/p-coumaroyl esterase activity when grown on wheat bran in solid-state culture.
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ISSN:0959-3993
1573-0972
DOI:10.1007/BF00704639