CSF-enriched antibodies do not share specificities among MS patients

The specificity of the oligoclonal immunoglobulins found in MS CSF is unknown. We have previously shown that random peptide libraries displayed on phage can be used to identify specific ligands for CSF antibodies. Here we describe the use of this tool in the attempt to identify MS-specific CSF-enric...

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Bibliographic Details
Published inMultiple sclerosis Vol. 4; no. 3; pp. 118 - 123
Main Authors Cortese, I, Capone, S, Luchetti, S, Grimaldi, L ME, Nicosia, A, Cortese, R
Format Journal Article
LanguageEnglish
Published Thousand Oaks, CA SAGE Publications 01.06.1998
Subjects
Adult
Antibodies - cerebrospinal fluid
Antibody Specificity
Bacteriophages
Female
Humans
Male
Middle Aged
Multiple Sclerosis - cerebrospinal fluid
Multiple Sclerosis - immunology
Peptide Library
phage library
multiple sclerosis
CSF
oligoclonal bands
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Summary:The specificity of the oligoclonal immunoglobulins found in MS CSF is unknown. We have previously shown that random peptide libraries displayed on phage can be used to identify specific ligands for CSF antibodies. Here we describe the use of this tool in the attempt to identify MS-specific CSF-enriched antibody reactivities with potential pathogenic, diagnostic or prognostic value. Applying different experimental strategies, several ligands reacting with CSF-enriched antibodies were identified. When tested against a panel of 55 MS patients, none of the ligands found were recognized by antibodies shared by any two patients. We used the selected peptides to demonstrate the stability in time of CSF-enriched antibodies notwithstanding disease progression.
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ISSN:1352-4585
1477-0970
DOI:10.1177/135245859800400305