Origin of platelet-derived growth factor in megakaryocytes in guinea pigs

Growth factor activity, as determined by the stimulation of [3H]thymidine incorporation into the DNA of quiescent 3T3 cells in culture, was found in lysates of guinea pig platelets and megakaryocytes. Quantitative dilution studies demonstrated that, of the cells present in the guinea pig bone marrow...

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Bibliographic Details
Published inThe Journal of clinical investigation Vol. 65; no. 4; pp. 926 - 930
Main Authors Chernoff, A, Levine, R F, Goodman, D S
Format Journal Article
LanguageEnglish
Published United States 01.04.1980
Subjects
Animals
Blood Platelets - cytology
Blood Platelets - metabolism
Cell Count
Growth Substances - biosynthesis
Growth Substances - blood
Guinea Pigs
Megakaryocytes - cytology
Megakaryocytes - metabolism
Mitogens - biosynthesis
Mitogens - blood
Platelet Count
Platelet-Derived Growth Factor
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Summary:Growth factor activity, as determined by the stimulation of [3H]thymidine incorporation into the DNA of quiescent 3T3 cells in culture, was found in lysates of guinea pig platelets and megakaryocytes. Quantitative dilution studies demonstrated that, of the cells present in the guinea pig bone marrow, only the megakaryocyte possessed quantitatively significant growth factor activity. The amount of activity present in one megakaryocyte was equivalent to that present in 1,000-5,000 platelets, a value approximately comparable to the number of platelets shed from a single megakaryocyte. It is suggested that guinea pig platelet-derived growth factor has its origin in the megakaryocyte.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0021-9738
DOI:10.1172/JCI109747