Semicarbazide-sensitive amine oxidase activity and total nitrite and nitrate concentrations in serum: novel biochemical markers for type 2 diabetes?

• Published in: Acta diabetologica Vol. 46; no. 2; pp. 135 - 140
• Main Authors: Nunes, Sandra Fernanda, Figueiredo, Isabel Vitoria, Soares, Paulo João, Costa, Nuria Espriu, Lopes, Maria Celeste, Caramona, Maria Margarida
• Format: Journal Article
• Language: English
• Published: Milan Springer Milan 01.06.2009; Springer Nature B.V
• Subjects: Administration, Oral; Aged; Aged, 80 and over; Albuminuria - epidemiology; Amine Oxidase (Copper-Containing) - blood; Biochemistry; Biomarkers - blood; Blood Glucose - metabolism; Blood tests; Cholesterol - blood; Comparative studies; Diabetes; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - enzymology; Female; Glycated Hemoglobin A - metabolism; Humans; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - therapeutic use; Internal Medicine; Male; Medical prognosis; Medicine; Medicine & Public Health; Metabolic Diseases; Middle Aged; Nitrates - blood; Nitric Oxide - blood; Nitrites - blood; Original Article; Prognosis; Reference Values; Reproducibility of Results; Triglycerides - blood; Type 2 diabetes; Semicarbazide-sensitive amine oxidase; Total nitrite and nitrate; Oxidative stress; Nitric oxide
• ISSN: 0940-5429; 1432-5233; 1432-5233
• DOI: 10.1007/s00592-008-0070-7

The aim of this study was to evaluate the activity of semicarbazide-sensitive amine oxidase (SSAO) and the total nitrite and nitrate (NO x ) concentrations in serum from type 2 diabetic patients and control subjects in order to evaluate if they could be used as novel diabetic markers. We studied 38 type 2 diabetic patients and 35 control subjects. Serum samples from those subjects were evaluated by radiochemical methods for SSAO activity using 14 C-benzylamine. Serum NO x concentrations were obtained as an index of nitric oxide production by the Griess reaction. Serum SSAO activity was higher in type 2 diabetic patients than in control group and serum SSAO in type 2 diabetic correlated with age, serum creatinine and total cholesterol. Serum NO x levels in type 2 diabetic patients were also significantly higher than those in the control group. Serum NO x levels in control group correlated with serum SSAO activity. In conclusion, the increase in the SSAO activity and NO x levels observed in type 2 diabetic patients could be parameters to take in account and play relevant role in diabetes development. SSAO and NO x are suggested as markers for prognostic of diabetes.