Amphetamines in Human Urine: Rapid Estimation by Gas--Liquid Chromatography

Abstract A procedure is described for the quantitative determination of amphetamine and p-methoxyamphetamine and for the semiquantitative determination of methamphetamine, mephentermine, and normephentermine in human urine. An internal reference compound, phenethylamine, is added to 5 ml of urine. T...

Full description

Saved in:
Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 16; no. 9; pp. 786 - 788
Main Authors Schweitzer, J. W, Friedhoff, A. J
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.09.1970
Subjects
Acetates
Amphetamine - urine
Anhydrides
Chloroform
Chromatography, Gas
Humans
Hydrogen-Ion Concentration
Mephentermine - urine
Methamphetamine - urine
Methods
Phenethylamines - urine
Psychoses, Substance-Induced - diagnosis
Online AccessGet full text

Cover

More Information
Summary:Abstract A procedure is described for the quantitative determination of amphetamine and p-methoxyamphetamine and for the semiquantitative determination of methamphetamine, mephentermine, and normephentermine in human urine. An internal reference compound, phenethylamine, is added to 5 ml of urine. The urine is extracted at low pH to remove acidic contaminants, then at high pH with chloroform, in which the amines were acetylated with acetic anhydride. After evaporation, the residue, dissolved in dioxane, is injected onto a column at 215°C, packed with 8% ethyleneglycol adipate on Chromosorb W, 100-120 mesh, except at both ends, where the packing was 8% silicon rubber SE 30 on Chromosorb W, 100-120 mesh. The excretion rate of amphetamines by patients admitted with a diagnosis of amphetamine psychosis was measured.
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/16.9.786