A new inhibitor of plasmin and trypsin from porcine leukocytes

• Published in: Biological chemistry Hoppe-Seyler Vol. 371; no. 1; p. 57
• Main Authors: Drobnic-Kosorok, M, Kopitar, M, Babnik, J, Jerala, R, Turk, V
• Format: Journal Article
• Language: English
• Published: Germany 01.01.1990
• Subjects: Amino Acid Sequence; Animals; Aprotinin; Cattle; Fibrinolysin - antagonists & inhibitors; Leukocytes - enzymology; Molecular Sequence Data; Swine; Trypsin Inhibitors - analysis; Trypsin Inhibitors - isolation & purification
• ISSN: 0177-3593
• DOI: 10.1515/bchm3.1990.371.1.57

A new intracellular inhibitor of plasmin and trypsin was isolated from porcine leukocytes by ion exchange chromatography and affinity chromatography. In dodecyl sulphate gel electrophoresis a single protein band with an apparent molecular mass of 15 kDa was found under reducing conditions. On isoelectric focusing three protein bands with isoelectric points between pH 4.0 and 4.5 were found. The association rate constants and the inhibition constants were determined for porcine plasmin and bovine trypsin. The inhibitor shows no immunologic cross-reactivity with any of the tested leukocyte inhibitors. On the basis of its N-terminal amino-acid sequence a great degree of similarity to Kunitz-type inhibitors was observed.