Radioimmunoassay of bile acids in serum

We developed four radioimmunoassay procedures for the determination of glycine-conjugated bile acids in serum. Antibodies to two primary bile acids, cholylgycine and chenodeoxycholylglycine, and to two secondary bile acids, sulfolithocholylglycine and deoxycholylglycine, were raised in rabbits after...

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Published inClinical chemistry (Baltimore, Md.) Vol. 22; no. 5; pp. 602 - 606
Main Authors Demers, LM, Hepner, G
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.05.1976
Subjects
Bile Acids and Salts - blood
Cholestasis - blood
Cross Reactions
Hepatitis - blood
Humans
Liver Cirrhosis - blood
Liver Neoplasms - blood
Microchemistry
Radioimmunoassay - methods
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Summary:We developed four radioimmunoassay procedures for the determination of glycine-conjugated bile acids in serum. Antibodies to two primary bile acids, cholylgycine and chenodeoxycholylglycine, and to two secondary bile acids, sulfolithocholylglycine and deoxycholylglycine, were raised in rabbits after the acids were covalently linked to albumin by use of the carbodiimide reaction. Assay sensitivity for each of these bile acids is in the picomole range with the standard curve extending from 10-80 pmol. The concentration of bile acids in serum increased in various states of liver disease and its measurement appears to be an extremely sensitive indicator of liver function.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/22.5.602