Effect of the air supply on the production of 2,3-butanediol by Klebsiella pneumoniae NRRL B199
Klebsiella pneumoniae was cultivated on sucrose in batch, under five quite different oxygen transfer conditions. Depending on the oxygen supply, three different phases could occur. With oxygen in solution, cell growth is exponential and butanediol/acetoin production depends on the oxygen transfer co...
Saved in:
Published in | Journal of biotechnology Vol. 31; no. 1; pp. 93 - 102 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
1993
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Klebsiella pneumoniae was cultivated on sucrose in batch, under five quite different oxygen transfer conditions. Depending on the oxygen supply, three different phases could occur. With oxygen in solution, cell growth is exponential and butanediol/acetoin production depends on the oxygen transfer coefficient (
K
L
a). A higher
K
L
a can completely hinder the butanediol formation. When dissolved oxygen concentration is zero, the growth becomes linear and the product formation has its greatest specific rate (
μ
p) as a function of
K
L
a. The maximum
μ
p is dependent on the
K
L
a of each experiment. Below its maximum value,
μ
p is a function of the specific oxygen uptake rate (
Q
O
2
). Under a very low oxygen supply, cell growth is suppressed and diol/acetoin formation rates decrease. A theoretical interpretation of the results is proposed. |
---|---|
ISSN: | 0168-1656 1873-4863 |
DOI: | 10.1016/0168-1656(93)90139-E |