A comparison of eosinophil-activating factor (EAF) with other monokines and lymphokines

Monocytes from moderately eosinophilic individuals secrete material that enhances the cytotoxic activity of eosinophils against antibody-coated schistosomula of Schistosoma mansoni. This material is not a single substance, but can be fractionated into several active components of different size and...

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Bibliographic Details
Published inEuropean journal of immunology Vol. 16; no. 9; p. 1143
Main Authors Thorne, K J, Richardson, B A, Taverne, J, Williamson, D J, Vadas, M A, Butterworth, A E
Format Journal Article
LanguageEnglish
Published Germany 01.09.1986
Subjects
Colony-Stimulating Factors - pharmacology
Eosinophils - drug effects
Glycoproteins - pharmacology
Humans
Interferon Type I - pharmacology
Interleukin-1
Interleukin-2
Lymphokines - isolation & purification
Lymphokines - pharmacology
Monocytes - analysis
Monokines
Proteins - pharmacology
Recombinant Proteins - pharmacology
Tumor Necrosis Factor-alpha
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Summary:Monocytes from moderately eosinophilic individuals secrete material that enhances the cytotoxic activity of eosinophils against antibody-coated schistosomula of Schistosoma mansoni. This material is not a single substance, but can be fractionated into several active components of different size and different charge. Gel filtration of mononuclear cell supernatants separated the eosinophil-activating activity into a major component of molecular mass of 40 kDa and a minor component of molecular mass of less than 10 kDa. The major component exhibited further heterogeneity on fractionation by high performance liquid chromatography. The bulk of the eosinophil-activating activity could be separated from both colony-stimulating factor (CSF) alpha activity and from tumor necrosis factor (TNF) activity. However, human recombinant CSF alpha (GM-CSF), human recombinant TNF and rabbit tumor necrosis serum all had eosinophil-activating activity when tested against schistosomula. Eosinophils were not activated by interleukin 1, interleukin 2, interferon-alpha, lipopolysaccharide or phorbol myristate acetate.
ISSN:0014-2980
DOI:10.1002/eji.1830160919