Experimental galactose toxicity: effects on synaptosomal phosphatidylinositol metabolism

Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2-9 weeks post-weaning and incubated with [33P][i and myo-[2-3H]inositol in the presence or absence of 0.2 mM-acetylcholine. The acetylcholine-stimula...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 37; no. 4; p. 888
Main Authors Berry, G, Yandrasitz, J R, Segal, S
Format Journal Article
LanguageEnglish
Published England 01.10.1981
Subjects
Acetylcholine - pharmacology
Animals
Brain - drug effects
Brain - metabolism
Dietary Carbohydrates - pharmacology
Galactose - toxicity
Phosphatidylinositols - metabolism
Rats
Rats, Inbred Strains
Synaptosomes - drug effects
Synaptosomes - metabolism
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Summary:Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2-9 weeks post-weaning and incubated with [33P][i and myo-[2-3H]inositol in the presence or absence of 0.2 mM-acetylcholine. The acetylcholine-stimulated [33P]Pi labeling of phosphatidylinositol and the changes in amounts of phosphatidylinositol were similar in the normal and galactose-toxic rats; however, acetylcholine-stimulated myo-[2-3H]inositol labeling of phosphatidylinositol was markedly decreased in the galactose-toxic rats. The impairment of acetylcholine-stimulated myo-[2-3H]inositol incorporation into phosphatidylinositol observed after 2 weeks on the diet did not vary after more prolonged exposure to galactose.
ISSN:0022-3042
DOI:10.1111/j.1471-4159.1981.tb04475.x