Demands and technical developments of clinical flow cytometry with emphasis in quantitative, spectral, and imaging capabilities

• Published in: Na mi ji shu yu jing mi gong cheng Vol. 5; no. 4; pp. 46 - 55
• Main Authors: Zhang, Ting, Gao, Mengge, Chen, Xiao, Gao, Chiyuan, Feng, Shilun, Chen, Deyong, Wang, Junbo, Zhao, Xiaosu, Chen, Jian
• Format: Journal Article
• Language: English
• Published: State Key Laboratory of Transducer Technology,Aerospace Information Research Institute,Chinese Academy of Sciences,Beijing 100190,People's Republic of China 01.12.2022; School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,People's Republic of China%Peking University People's Hospital,Peking University Institute of Hematology,National Clinical Research Center for Hematologic Disease,Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation,Beijing 100044,People's Republic of China%State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,People's Republic of China%State Key Laboratory of Transducer Technology,Aerospace Information Research Institute,Chinese Academy of Sciences,Beijing 100190,People's Republic of China; School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,People's Republic of China; School of Electronic,Electrical and Communication Engineering,University of Chinese Academy of Sciences,Beijing 100049,People's Republic of China; AIP Publishing LLC
• Subjects: Quantitative flow cytometry; Spectral flow cytometry; Clinical flow cytometry; Imaging flow cytometry; Clinical demand
• ISSN: 1672-6030; 2589-5540; 2589-5540
• DOI: 10.1063/10.0015301

As the gold-standard method for single-cell analysis, flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells. This review highlights the demands for clinical flow cytometry in laboratory hematology (e.g., diagnoses of minimal residual disease and various types of leukemia), summarizes state-of-the-art clinical flow cytometers (e.g., FACSLyricTM by Becton Dickinson, DxFLEX by Beckman Coulter), then considers innovative technical improvements in flow cytometry (including quantitative, spectral, and imaging approaches) to address the limitations of clinical flow cytometry in hematology diagnosis. Finally, driven by these clinical demands, future developments in clinical flow cytometry are suggested.