Short-term effectiveness of transcranial direct current stimulation in the nociceptive behavior of neuropathic pain rats in development

• Published in: AIMS neuroscience Vol. 10; no. 4; pp. 433 - 446
• Main Authors: Crespo, Priscila Centeno, Anderson Meira Martins, Leo, Martins, Otávio Garcia, Camacho Dos Reis, Clara, Goulart, Ricardo Netto, de Souza, Andressa, Medeiros, Liciane Fernandes, Scarabelot, Vanessa Leal, Gamaro, Giovana Duzzo, Silva, Sabrina Pereira, de Oliveira, Marcos Roberto, Torres, Iraci Lucena da Silva, de Souza, Izabel Cristina Custódio
• Format: Journal Article
• Language: English
• Published: United States AIMS Press 01.01.2023
• Subjects: brain derived neurotrophic factor; development; neuropathic pain; noninvasive brain stimulation; transcranial direct current stimulation; brain derived neurotrophic factor; development; neuropathic pain; transcranial direct current stimulation; noninvasive brain stimulation
• ISSN: 2373-7972; 2373-7972
• DOI: 10.3934/Neuroscience.2023032

Neuropathic pain (NP) is caused by a lesion that triggers pain chronification and central sensitization and it can develop in a different manner, dependent of age. Recent studies have demonstrated the efficacy of transcranial direct current stimulation (tDCS) for treating NP. Then, we aimed to investigate the effects of tDCS and BDNF levels in neuropathic pain rats in development, with 30 days old in the beginning of experiments. Eight-five male rats were subjected to chronic constriction injury. After establishment of NP, bimodal tDCS was applied to the rats for eight consecutive days, for 20 minutes each session. Subsequently, nociceptive behavior was assessed at baseline, 14 days after surgery, 1 day and 7 days after the end of tDCS. The rats were sacrificed 8 days after the last session of tDCS. An increase in the nociceptive threshold was observed in rats in development 1 day after the end of tDCS (short-term effect), but this effect was not maintained 7 days after the end of tDCS (long-term effect). Furthermore, brain derived neurotrophic factor (BDNF) levels were analyzed in the frontal cortex, spinal cord and serum using ELISA assays. The neuropathic pain model showed an effect of BDNF in the spinal cord of rats in development. There were no effects of BNDF levels of pain or tDCS in the frontal cortex or serum. In conclusion, tDCS is an effective technique to relieve nociceptive behavior at a short-term effect in neuropathic pain rats in development, and BDNF levels were not altered at long-term effect.