Carbon nanomaterial-treated cell cultures of Nostoc flagelliforme produce exopolysaccharides with ameliorative physio-chemical properties

• Published in: International journal of biological macromolecules Vol. 227; pp. 726 - 735
• Main Authors: Yuan, Xiaolong, Gao, Xiang, Zheng, Tao, Wang, Jing, Dong, Yibei, Xue, Huidan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2023
• Subjects: Antioxidant activity; Carbon nanomaterials; Cell Culture Techniques; Chemical Phenomena; Cyanobacteria; Exopolysaccharide; Hydrogen Peroxide; Nanotubes, Carbon; Nostoc; Physio-chemical property; Polysaccharides - metabolism; Physio-chemical property; Exopolysaccharide; Antioxidant activity; Cyanobacteria; Carbon nanomaterials
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.12.209

The feasibility and efficiency of carbon nanomaterials (CNMs) in algal biotechnology are less known. In this study, the influences of four CNMs, graphene (G), graphene oxide (GO), multiwalled carbon nanotube (MWCNT), and aminated multiwalled carbon nanotube (MWCNT-NH2), on cell growth and exopolysaccharide (EPS) production, as well as the physiochemical properties of EPS, were investigated in cell culture of Nostoc flagelliforme. A proper concentration (15 mg L−1) of four CNMs was chosen for use after a preliminary test. Upon GO treatment, the biomass was improved by 11.1 % and the EPS production was increased by 36.1 % on day 16 compared to the nontreated control. Four CNM treatments significantly improved cellular O2·− and H2O2 levels as well as superoxide dismutase and catalase activities. The monosaccharide compositions and functional groups of the EPSs were obviously altered by the CNM treatments. Particularly, the GO treatment-resulting EPS showed obviously improved flocculating ability, water absorption ability, and reactive oxygen species scavenging ability. In general, four CNMs exerted distinct influences on the production and physio-chemical property alteration of the EPS in N. flagelliforme culture. This work expands our understanding of the application of CNMs in the induced production and functional modification of polysaccharides during algal cultivation.