Identification of novel laccase from cyanobacterium Microcystis flos-aquae and enhanced azo dye bioremediation potential

• Published in: Bioresource technology Vol. 399; p. 130587
• Main Authors: Muhammad, Muhammad Ayyub, Balogun, Emmanuel Oluwadareus, Sallau, Abdullahi Balarabe, Chia, Mathias Ahii, Shuaibu, Mohammed Nasir
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2024
• Subjects: 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid; Cibacron Brilliant Blue; Cyanobacteria; Decolorization; Green Oxidation; Recalcitrant Organic Pollutants; Green Oxidation; Recalcitrant Organic Pollutants; Cibacron Brilliant Blue; 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid; Decolorization; Cyanobacteria
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2024.130587

[Display omitted] •Identification of a novel extracellular laccase in Microcystis flos-aquae.•Enhanced bioremediation potential for azo dyes.•Successful rapid decolorization of Cibacron Brilliant Blue.•Introduction of an eco-friendly green oxidation method.•Laccase activity was effective across a wide pH spectrum.•The bioremediation process was efficient at various temperatures. Textile industries discharge up to 280,000 tons of dye waste annually, resulting in global pollution and health risks. In Nigeria and other African countries, persistent dyes threaten aquatic life and human health. This study introduces a cost-effective, enzyme-mediated bioremediation alternative using a novel laccase from the cyanobacteriumMicrocystis flos-aquae. This purified enzyme yielded 0.55 % (w/w)with significant activity at 40 °C and pH 4.00. Kinetic studies showed the dependence of M. flos-aquae laccase on Cu2+and its inhibition by EDTA and Fe2+. The efficacy of the enzyme was demonstrated through rapid decolorization of the azo dye Cibacron Brilliant Blue over a wide temperature and pH range. As this enzyme effectively decolorizes dyes across a broad temperature and pH range, it offers a promising solution for bioremediation of textile effluents.