Structural Basis for the Sequence Selectivity of DNA Cleavage by Bleomycins

DNA strand scission by the bleomycin analogs talisomycin S 10b and a novel fluorescent mimic, fluoromycin, has been characterized and compared with that of bleomycin A 2. Both analogs were found to have essentially the same sequence specificity for DNA strand scission as bleomycin A 2. As observed w...

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Published inBiochemical and biophysical research communications Vol. 191; no. 2; pp. 420 - 426
Main Authors Mistry, J.S., Koepsel, R.R., Lazo, J.S.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.03.1993
Elsevier
Subjects
Antineoplastic agents
Base Sequence
Binding Sites
Biological and medical sciences
Bleomycin - chemistry
Bleomycin - pharmacology
Carbohydrate Sequence
DNA - drug effects
Fluorescent Dyes
General aspects
Medical sciences
Molecular Sequence Data
Pharmacology. Drug treatments
Plasmids
Structure-Activity Relationship
Antineoplastic agent
Sequence specificity
Antibiotic
Structure activity relation
DNA
Mechanism of action
In vitro
Comparative study
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Summary:DNA strand scission by the bleomycin analogs talisomycin S 10b and a novel fluorescent mimic, fluoromycin, has been characterized and compared with that of bleomycin A 2. Both analogs were found to have essentially the same sequence specificity for DNA strand scission as bleomycin A 2. As observed with bleomycin A 2, the preferred sites of DNA cleavage by talisomycin S 10b and fluoromycin were 5′-G pT-3′ and 5′-G pC-3′. These results suggest that the DNA sequence selectivity of bleomycins remains unaltered upon modification of the C-terminal domain.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.1234