LC-MS/MS analysis of partial structure of Panax ginseng protein and its distribution in vivo

Protein from Panax ginseng can improve learning, memory, and analgesia. Here, we investigated a fluorescence labeling method that can be used to determine the in vivo distribution of P. ginseng protein (PGP). High-performance liquid chromatography (HPLC) was used to define the amino acid composition...

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Bibliographic Details
Published inInternational journal of biological macromolecules Vol. 150; pp. 695 - 704
Main Authors Fang, Xiaoxue, Zhu, Difu, Chen, Yinghong, Song, Lianlian, Jiang, Ruizhi, Shan, Mengyao, Qiu, Zhidong, Luo, Haoming
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.05.2020
Subjects
Animals
Chromatography, Liquid
Fluorescence labeling
Ginseng protein
Mice
Panax - chemistry
Plant Proteins - chemistry
Plant Proteins - pharmacokinetics
Plant Proteins - pharmacology
Protein Conformation
Tandem Mass Spectrometry
Tissue distribution
UV
Ginseng protein
BBB
BDNF
EGF
ACTH
NT-3
IR
IGF
TFA
Te
FITC
Fluorescence labeling
PGP
Tissue distribution
HPLC
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Summary:Protein from Panax ginseng can improve learning, memory, and analgesia. Here, we investigated a fluorescence labeling method that can be used to determine the in vivo distribution of P. ginseng protein (PGP). High-performance liquid chromatography (HPLC) was used to define the amino acid composition and molecular weight of PGP; LC-MS/MS was used to identify the PGP structure, which was fluorescently-labeled using a fluorescein isothiocyanate (FITC) probe. The connection form of the PGP fluorescent marker (PGP-FITC) was identified by ultraviolet and infrared spectrophotometry. The in vivo distribution of PGP was observed by fluorescence imaging, and tissue content was determined. Results showed that PGP was enriched in the brain and that vascular epithelial cells showed specific uptake. We provide an experimental method to label and identify the in vivo distribution of PGP, which forms the basis for future studies to determine whether PGP can penetrate the blood-brain barrier (BBB) and elucidate the transport mechanism.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.02.123