Metabolomic analysis of the effects of a commercial complex biostimulant on pepper crops

• Published in: Food chemistry Vol. 310; p. 125818
• Main Authors: Barrajón-Catalán, Enrique, Álvarez-Martínez, Francisco Javier, Borrás, Fernando, Pérez, David, Herrero, Noemí, Ruiz, Juan J., Micol, Vicente
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 25.04.2020
• Subjects: Acylglycerol; Biostimulant; Capsianoside; Capsicum - drug effects; Capsicum - metabolism; Carotenoid; Carotenoids - metabolism; Chromatography, High Pressure Liquid; Crops, Agricultural - metabolism; Fruit - drug effects; Fruit - metabolism; Fruit - physiology; HPLC-ESI-QTOF-MS; Metabolome; Metabolomics; Monosaccharides - metabolism; Pepper; Phenylalanine - metabolism; Plant Growth Regulators - pharmacology; Plant Leaves - drug effects; Plant Leaves - metabolism; Plant Leaves - physiology; Ripening; Spectrometry, Mass, Electrospray Ionization; Ripening; Metabolomics; HPLC-ESI-QTOF-MS; Pepper; Capsianoside; Acylglycerol; Biostimulant; Carotenoid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2019.125818

•Biostimulants are increasing in use and importance for agriculture and food production globally.•A metabolomic approach elucidated biostimulant action on pepper plants.•The tested biostimulant (Actium®) modified compounds in pepper fruits and leaves. Research on plant biostimulants is of interest in their potential benefits for agriculture production and environmental sustainability. These naturally occurring products induce beneficial consequences in plant metabolism and productivity. In most cases their modes of action, and consequences for the whole plant as well as parts, such as the fruit, are well characterized, but the precise mechanisms of action require further attention. This study examined the effects of the commercial biostimulant, Actium®, on Capsicum annuum L. cv Palermo leaves and fruits. The influence of time (characterized by ripening), after 14 and 28 days of treatment, treatment regimen, and their combined impact on the metabolome were studied using HPLC-ESI-QTOF-MS analysis of polar and apolar compounds. The results showed that flavonoids and capsianosides decreased with ripening in leaves, but organic acids, monosaccharides, and carotenoids increased in fruits. The treatment of Capsicum fruits with Actium® increased phenylalanine and total monosaccharides (glucose and fructose) compared to controls, suggesting a further stage in ripening. An increase in carotenoids concomitant with an increase of some digalactosyl diacylglycerols, which are part of the chromoplasts lipid machinery of enzymes involved in the synthesis of carotenoids, was also observed. Our findings suggest that this biostimulant may increase some metabolites related to pepper fruit maturity and coloration in pepper crops.