Determination of diffusion coefficient for released nanoparticles from developed gelatin/chitosan bilayered buccal films

This study aims at the mathematical optimization by Box-Behnken statistical design, fabrication by ionic gelation technique and in vitro characterization of insulin nanoparticles containing thiolated N- dimethyl ethyl chitosan (DMEC-Cys) conjugate. Then Optimized insulin nanoparticles were loaded in...

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Bibliographic Details
Published inInternational journal of biological macromolecules Vol. 112; pp. 1005 - 1013
Main Authors Mahdizadeh Barzoki, Zahra, Emam-Djomeh, Zahra, Mortazavian, Elaheh, Rafiee-Tehrani, Niyousha, Behmadi, Homa, Rafiee-Tehrani, Morteza, Moosavi-Movahedi, Ali Akbar
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2018
Subjects
Buccal film
Diffusion coefficient
Nanoparticles
Optimization
Oral drug delivery
Toxicology
Nanoparticles
Toxicology
Oral drug delivery
Buccal film
Diffusion coefficient
Optimization
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Summary:This study aims at the mathematical optimization by Box-Behnken statistical design, fabrication by ionic gelation technique and in vitro characterization of insulin nanoparticles containing thiolated N- dimethyl ethyl chitosan (DMEC-Cys) conjugate. Then Optimized insulin nanoparticles were loaded into the buccal film, and in-vitro drug release from films was investigated, and diffusion coefficient was predicted. The optimized nanoparticles were shown to have mean particle size diameter of 148nm, zeta potential of 15.5mV, PdI of 0.26 and AE of 97.56%. Cell viability after incubation with optimized nanoparticles and films were assessed using an MTT biochemical assay. In vitro release study, FTIR and cytotoxicity also indicated that nanoparticles made of this thiolated polymer are suitable candidates for oral insulin delivery.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2018.01.215