An effect of choline lactate based low transition temperature mixtures on the lipase catalytic properties

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 216; p. 112518
• Main Authors: Hoppe, Jakub, Byzia, Ewa, Drozd, Radosław, Szymańska, Magdalena, Bielecki, Patryk, Smiglak, Marcin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2022
• Subjects: Hydrolytic activity thermal stability; Ionic liquids; Lipase; Low transition temperature mixtures; Transesterification activity; LTTM; DES; Ionic liquids; CALB; Lipase; Low transition temperature mixtures; Hydrolytic activity thermal stability; Transesterification activity
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2022.112518

A new series of low transition temperature mixures (LTTM) based on choline lactate quaternary ammonium salt and various hydrogen bond donors was prepared and characterized towards their physicochemical properties and usability as an enzymatic reaction mixture for lipase-catalyzed transesterification reactions. Studies of low transition temperature mixtures have shown a long-term stabilizing effect for lipase as well as a positive influence on lipase thermal stability. In the case of Ch[Lac]:Gly: EthGly increasing the stability of lipase by 8 °C (up to 55.2 °C) compared to the control sample. Conducted transesterification reactions were characterized by high yields – up to 98% – and high purity of the obtained products. [Display omitted] •A new set of choline lactate-based LTTMs was developed.•The effect of choline lactate LTTMs on lipase stability and activity was investigated.•The operational parameters of lipase depend on LTTMs composition.•The LTTMs based on choline lactate are useful media for transesterification catalyzed by lipase.