Promoter Binding Proteins of an Active Human L1 Retrotransposon

• Published in: Biochemical and biophysical research communications Vol. 191; no. 2; pp. 625 - 632
• Main Authors: Mathias, S.L., Scott, A.F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.03.1993; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Base Sequence; Binding and carrier proteins; Biological and medical sciences; DNA; DNA Transposable Elements; Fundamental and applied biological sciences. Psychology; HeLa Cells; Humans; Molecular Sequence Data; Neoplasm Proteins - genetics; Promoter Regions, Genetic; Proteins; Repetitive Sequences, Nucleic Acid; Transcription, Genetic; Tumor Cells, Cultured; Human; High repeated sequence; Interspersed repeated sequence; Retrotransposon; Cell line; Transcription promoter; DNA binding protein; Footprinting technique; Molecular interaction; Malignant teratoma
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1993.1263

Human Long Interspersed elements (LINEs or L1 Hs elements) are a highly repetitive class of DNA sequences which are dispersed by retrotransposition. Full length L1 Hs transcripts and L1-encoded proteins have been shown by others to be present in NTera2Dl and other related teratocarcinoma cell lines. Using standard methods for the identification of DNA binding proteins we identified two proteins or complexes of proteins which specifically bind to the L1 Hs promoter region and are qualitatively different between NTera2D1 and HeLa cell nuclear extracts. These proteins represent candidates for the factors controlling the differential expression of L1 sequences.