A quantitative proteomic analyses of primary myocardial cell injury induced by heat stress in chicken embryo

• Published in: Journal of thermal biology Vol. 112; p. 103461
• Main Authors: Wang, Rui, Guo, Yanli, Shi, Zhaoguo, Qin, Shizhen
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2023
• Subjects: Animals; Apoptosis; Chick Embryo; Chickens - metabolism; Heat stress; Heat-Shock Response; Metabolic Networks and Pathways; Oxidative stress; Primary chick embryonic myocardial cells; Proteome - metabolism; Proteomics; Proteomics - methods; Oxidative stress; Heat stress; Primary chick embryonic myocardial cells; Proteomics; Apoptosis
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/j.jtherbio.2023.103461

In this study, the model of heat stress was constructed in primary chick embryonic myocardial cells at 42 °C for 4 h. Proteome analysis using DIA identified 245 differentially expressed proteins (DEPs) (Q-value <0.05, fold change >1.5), of which 63 proteins were up-regulated and 182 proteins were down-regulated. Many were related to metabolism, oxidative stress, oxidative phosphorylation and apoptosis. Gene Ontology (GO) analysis showed that many DEPs under heat stress were involved in regulating metabolites and energy, cellular respiration, catalytic activity and stimulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEPs were enriched in metabolic pathways, oxidative phosphorylation, citrate cycle (TCA cycle), cardiac muscle contraction, and carbon metabolism. The results could help understanding of the effect of heat stress on myocardial cells and even the heart and possible action mechanism at the protein level. •245 differentially expressed proteins (DEPs) were identified and many were related to metabolism, oxidative stress, oxidative phosphorylation and apoptosis.•The electron transfer and REDOX reaction in the respiratory chain were affected.