An in-vitro evaluation of coralline porous hydroxyapatite as a scaffold for osteoblast growth

• Published in: Clinical materials Vol. 17; no. 2; pp. 85 - 91
• Main Authors: Norman, Maria E., Elgendy, Hoda M., Shors, Edwin C., El-Amin, Saadiq F., Laurencin, Cato T.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 1994; Oxford Elsevier
• Subjects: Biocompatible Materials; Biological and medical sciences; Cell Division; Cell Line; Ceramics; Durapatite; Health technology assessment; Humans; In Vitro Techniques; Medical sciences; Microscopy, Electron, Scanning; Osseointegration - physiology; Osteoblasts - cytology; Osteoblasts - physiology; Porosity; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Technology. Biomaterials. Equipments. Material. Instrumentation; Biological properties; Evaluation; Growth; Biological medium; Calcium phosphate; Hydroxyapatite; Bone; Cell; Ceramic materials; In vitro; Osteogenesis; Biomedical engineering
• ISSN: 0267-6605; 1878-6979
• DOI: 10.1016/0267-6605(94)90016-7

The purpose of this study was to determine the potential of coralline calcium phosphate ceramics to support osteoblast growth for a proposed boneceramic cerposite for skeletal tissue repair. The goal was the development of a matrix with both osteogenic and osteoconductive properties, as compared to ceramic alone, which is solely osteoconductive. MC3T3-E1 osteoblast-like cells were seeded onto sintered and non-sintered porous coralline hydroxyapatite (HA), and onto non-porous hydroxyapatite discs. These in-vitro studies demonstrated that coralline HA supported the growth of osteoblast-like cells. Porous discs supported higher numbers of cells than non-porous discs. Sintering encouraged cell growth, with higher numbers of cells adhered to sintered porous HA discs by day seven. The results suggest that HA can provide a support for osteoblast cells as part of a matrix which may prove to be osteogenic in vivo and may, accordingly, enhance the bone repair process.