Biomass production of Chlorella pyrenoidosa by filled sphere carrier reactor: Performance and mechanism

• Published in: Bioresource technology Vol. 383; p. 129195
• Main Authors: Wei, Sijing, Li, Fei, Zhu, Nengwu, Wei, Xiaorong, Wu, Pingxiao, Dang, Zhi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2023
• Subjects: Biomass; Carbon; Carbon Dioxide; Carbon Sequestration; Chlorella; Chlorella pyrenoidosa; CO2 fixation; Electron transfer; Filled sphere carrier reactor; High biomass production; Microalgae; Electron transfer; Chlorella pyrenoidosa; Filled sphere carrier reactor; CO2 fixation; High biomass production; CO fixation
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129195

[Display omitted] •Filled sphere polyester carrier reactor was proposed for C. pyrenoidosa growth.•Dry biomass yield reached 9.98 g/L and 249.5 folds higher than suspension control.•7% flue gas CO2 further increased carbon sequestration rate to 18.32 g/L/d.•Rise of nitrogen and phosphorus triggered electron transfer and metabolic process.•Increase of RuBisCO and CA enzyme activities intensified PSI and PSII process. Microalgae-based Carbon Capture, Utilization and Storage is vital for mitigating global climate change. A filled sphere carrier reactor was developed to achieve high biomass production and carbon sequestration rate of Chlorella pyrenoidosa. By introducing air (0.04% CO2) into the reactor, the dry biomass production achieved 8.26 g/L with the optimized parameters of polyester carrier, 80% packing density, 5-fold concentrated nutrient combining 0.2 mol/L phosphate buffer. At simulated flue gas CO2 concentration of 7%, the dry biomass yield and carbon sequestration rate reached up to 9.98 g/L and 18.32 g/L/d in one day, which were as high as 249.5 and 79.65 times comparing with those of suspension culture at day 1, respectively. The mechanism was mainly attributed to the obvious intensification of electron transfer rate and remarkable increase of RuBisCO enzyme activity in the photosynthetic chloroplast matrix. This work provided a novel approach for potential microalgae-based carbon capture and storage.