Activation by Protein Synthesis Inhibitors of Glucose Transport into L6 Muscle Cells

The effects of protein synthesis inhibitors on glucose transport into L6 myotubes was examined. Similar to the acute effects of insulin, short-term treatment with anisomycin or cycloheximide increased transport 2-fold. Kinetic studies demonstrated that this activation was due to a doubling in the V...

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Published inBiochemical and biophysical research communications Vol. 190; no. 3; pp. 881 - 887
Main Authors Hayes, N., Biswas, C., Strout, H.V., Berger, J.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.02.1993
Elsevier
Subjects
Animals
Anisomycin - pharmacology
Biological and medical sciences
Biological Transport - drug effects
Cell physiology
Cells, Cultured
Cycloheximide - pharmacology
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
In Vitro Techniques
Insulin - pharmacology
Membrane and intracellular transports
Mice
Molecular and cellular biology
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
Muscles - drug effects
Muscles - metabolism
Protein Synthesis Inhibitors - pharmacology
Cell line
Glucose
Protein synthesis inhibitor
Myotube
Striated muscle
Membrane translocation
Carrier protein
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Summary:The effects of protein synthesis inhibitors on glucose transport into L6 myotubes was examined. Similar to the acute effects of insulin, short-term treatment with anisomycin or cycloheximide increased transport 2-fold. Kinetic studies demonstrated that this activation was due to a doubling in the V max values. The effects of anisomycin or cycloheximide were not additive to that of insulin. Both protein synthesis inhibitors caused only small increases in GLUT4 expression and negligible effects on GLUT1 expression. Anisomycin, as well as insulin, induced the translocation of both transporters from intracellular vesicles to the plasma membrane though not in quantities sufficient to entirely account for the activation of transport. The results indicate that protein synthesis inhibitors stimulate hexose transport in L6 myotubes by increasing the number of transporters in the plasma membrane and augmenting the intrinsic catalytic activity of the transporters.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.1131