Poly-l-lysine-modified reduced graphene oxide stabilizes the copper nanoparticles with higher water-solubility and long-term additively antibacterial activity

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 107; pp. 107 - 114
• Main Authors: Ouyang, Yu, Cai, Xiang, Shi, QingShan, Liu, Lili, Wan, Dongliang, Tan, Shaozao, Ouyang, Yousheng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2013
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Copper - pharmacology; Copper nanoparticles; Escherichia coli - cytology; Escherichia coli - drug effects; Escherichia coli - growth & development; Graphite - chemistry; Graphite - pharmacology; Long-term additively antibacterial activity; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microbial Sensitivity Tests; Microbial Viability - drug effects; Oxidation-Reduction; Oxides - pharmacology; Photoelectron Spectroscopy; Poly-l-lysine; Polylysine - chemical synthesis; Polylysine - chemistry; Polylysine - pharmacology; Reduced graphene oxide; Solubility; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus - cytology; Staphylococcus aureus - drug effects; Time Factors; Water - chemistry; Water-solubility; X-Ray Diffraction; Water-solubility; Reduced graphene oxide; Long-term additively antibacterial activity; Copper nanoparticles; Poly-l-lysine
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2013.01.073

[Display omitted] ► PLL–rGO–CuNPs dispersion was found to remain stable for more than 3 months. ► The solubility of PLL–rGO–CuNPs could reach up to 1.4mg/mL. ► The PLL–rGO–CuNPs showed long-term additively antibacterial effect. ► PLL–rGO–CuNPs could play a role by disrupting the ion concentrations of intracellular fluid. In order to improve the water-solubility and long-term antibacterial activity of copper nanoparticles (CuNPs), a poly-l-lysine-modified reduced graphene oxide (PLL–rGO) was used as the carrier of CuNPs, and a poly-l-lysine/reduced graphene oxide/copper nanoparticles (PLL–rGO–CuNPs) hybrid was prepared by anchoring the CuNPs on the reduced graphene oxide surface. The novel PLL–rGO–CuNPs hybrid was characterized and the antibacterial activity of it on gram-negative Escherichia coli and Gram-positive Staphylococcus aureus was tested. Such a hybrid showed additively antibacterial activity, and the CuNPs on PLL–rGO were more stable than those on polyvinyl pyrrolidone, resulting in long-term additively antibacterial effect. Meanwhile, this hybrid showed excellent water-solubility, suggesting great potential application in microbial control.